Seplife Pd oneS Plasmid DNA Ion-Exchange Medium, 75±10 µm (CAT#: STEM-BC-3074-ZJF)

This ion-exchange medium is designed for purification of plasmid DNA. It selectively binds plasmid DNA while RNA, endotoxin, host-cell proteins, and other impurities are removed during washing. The medium can separate open-circular plasmid forms from supercoiled plasmid DNA. This separation increases the concentration of the desired supercoiled form. Purified plasmid DNA can be precipitated with isopropanol and washed with 70% ethanol. The recovered DNA can be used for restriction digestion, PCR, sequencing, transformation, and related molecular-biology applications. Gravity-column formats support purification quantities from 0.5 to 10 mg. Larger gravity columns can process higher plasmid quantities while maintaining purification performance. The medium can be cleaned in place and reused under controlled conditions.

Model: Seplife ® Pd oneS
Appearance: White or off-white particles
Type: Ion-exchange chromatography medium
Matrix: Polystyrene-divinylbenzene
Ligand: Quaternary amine
Median particle size d50v: 75±10 µm
Long-term pH stability: 4-12
Short-term CIP pH stability: 1-14
Chemical compatibility: Common buffers, 1 M sodium hydroxide, 8 M urea, 8 M guanidine hydrochloride, 75% ethanol, and 30% isopropanol
Gravity binding capacity:>0.25 mg/mL for high-copy plasmid at 2 cm bed height
Operating temperature: 4-25°C
Heat resistance: 40°C in water for 2 h
Gravity flow rate: ≥60 cm/h at 2 cm bed height
Gravity-column capacities: At least 0.5, 1, 5, and 10 mg depending on size

Specific plasmid DNA binding
One-step purification capability
Removes RNA and endotoxin
Separates supercoiled plasmid
Broad CIP pH range
Multiple gravity-column capacities
Reusable chromatography medium
Suitable for molecular-biology workflows

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