rProtein A Seplife Accel 50 Affinity Medium, 55-65 mg IgG/mL (CAT#: STEM-BC-3126-ZJF)

This Protein A affinity medium is designed for large-scale antibody manufacturing. It uses a rigid polymethyl methacrylate matrix with accurately controlled porosity. A long hydrophilic spacer arm reduces steric hindrance and nonspecific adsorption. Alkali-resistant recombinant Protein A selectively binds the Fc region of antibodies. The medium provides a dynamic human polyclonal IgG capacity of 55-65 mg/mL at five minutes residence time. Its 50 µm monodisperse particles support high resolution and stable packed-bed performance. The rigid matrix allows flow up to 800 cm/h and operating pressure up to 0.8 MPa. The medium is suitable for monoclonal and bispecific antibody capture with alkaline cleaning between cycles.

Model: rProtein A Seplife®Accel 50
Appearance: Milky-white spherical beads
Matrix: Polymethyl methacrylate
Ligand: Alkali-resistant recombinant Protein A
Median particle size d50: 50 µm
Dynamic binding capacity: 55-65 mg human polyclonal IgG/mL at 5 min residence time
Maximum pressure: 0.8 MPa
Recommended flow: 100-800 cm/h
pH stability: 2-12
Alkali resistance: 0.1-0.5 M sodium hydroxide
Chemical compatibility: Common buffers, acetic acid, 1 M hydrochloric acid, ethanol, isopropanol, and compatible organic solvents
Operating temperature: Stated as approximately 2-40°C
Storage: 20% ethanol or benzyl alcohol at approximately 2-8°C

High antibody binding capacity
Alkali-resistant Protein A
Rigid high-pressure particles
Low operating backpressure
Hydrophilic spacer modification
High aggregate-removal efficiency
Up to 800 cm/h flow
Designed for large-scale antibody production

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