Seplife LXPM Ni 5504 IDA Affinity Medium, ≥25 mg His Protein/mL (CAT#: STEM-BC-3124-ZJF)

This immobilized-metal affinity medium is designed for purification of His-tagged recombinant proteins and compatible metal-binding molecules. It uses a polymethyl methacrylate matrix with a hydrophilic surface modification. IDA chelating groups immobilize nickel ions on the particle surface. Histidine-containing proteins bind selectively to the coordinated nickel during chromatography. The medium can also process compatible peptides, nucleotides, and phosphorylated proteins. Its 1000 Å pore structure supports transport of biological macromolecules into the particles. The rigid 80 µm particles provide high mechanical stability and recommended linear flow up to 800 cm/h. The stated His-protein capacity of at least 25 mg/mL supports preparative affinity purification.

Model: Seplife® LXPM Ni 5504 IDA
Matrix: Polymethyl methacrylate
Appearance: Light-blue opaque spherical particles
Chelating chemistry: IDA with Ni²⁺
Median particle size d50v: 80 µm
Pore size: 1000 Å
His-tagged protein capacity: ≥25 mg/mL
Recommended linear flow: 100-800 cm/h
Maximum pressure: 0.8 MPa
pH stability: 2-12
Storage solution: 20% ethanol with stated sodium chloride preservative
Chemical compatibility: 0.1 M sodium hydroxide, 0.1 M hydrochloric acid, 8 M urea, 6 M guanidine hydrochloride for 24 h, 30% isopropanol, 2% SDS, and 70% ethanol after nickel removal

High ≥25 mg/mL capacity
IDA nickel-chelating ligand
Selective His-tag binding
Hydrophilic low-binding surface
1000 Å porous structure
Up to 800 cm/h flow
Strong mechanical stability
Broad reagent compatibility

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