This dextran chromatography medium is designed for desalting, peptide purification, extract cleanup, and molecular-weight determination. It consists of dextran crosslinked with epichlorohydrin to form controlled pores. Molecules are separated according to their ability to enter the internal gel structure. Excluded large molecules pass through the spaces between particles and elute first. Intermediate molecules enter some pores and move through the column more slowly. Small molecules enter a greater proportion of the pore volume and elute later. The fine particle range supports improved separation efficiency. The stated molecular-weight fractionation range is 1500-30000 Da.
Specification
Model: Seplife G50 F Appearance: White spherical particles, odorless and tasteless Dry particle size: 20-80 µm Swelling volume: 9-11 mL/g dry powder Fractionation range: 1500-30000 Da Pressure behavior: Follows Darcy's law Flow behavior: Follows Darcy's law Long-term pH stability: 2-10 Short-term CIP pH stability: 2-13 Applications: Desalting, peptide separation, biological-extract cleanup, and molecular-weight determination
Features
Fine-particle separation 1500-30000 Da range Suitable for peptide purification Extract-cleanup capability Molecular-weight determination Controlled dextran pores Broad CIP pH stability Improved chromatographic efficiency