Seplife G50 F Dextran Size-Exclusion Medium, 20-80 µm (CAT#: STEM-BC-3185-ZJF)

Highlights

Fine-particle separation
1500-30000 Da range
Suitable for peptide purification

Cat Number: STEM-BC-3185-ZJF

Application: for fine-particle peptide and biomolecule separation

Model: Seplife G50 F




Description

This dextran chromatography medium is designed for desalting, peptide purification, extract cleanup, and molecular-weight determination. It consists of dextran crosslinked with epichlorohydrin to form controlled pores. Molecules are separated according to their ability to enter the internal gel structure. Excluded large molecules pass through the spaces between particles and elute first. Intermediate molecules enter some pores and move through the column more slowly. Small molecules enter a greater proportion of the pore volume and elute later. The fine particle range supports improved separation efficiency. The stated molecular-weight fractionation range is 1500-30000 Da.

Specification

Model: Seplife G50 F
Appearance: White spherical particles, odorless and tasteless
Dry particle size: 20-80 µm
Swelling volume: 9-11 mL/g dry powder
Fractionation range: 1500-30000 Da
Pressure behavior: Follows Darcy's law
Flow behavior: Follows Darcy's law
Long-term pH stability: 2-10
Short-term CIP pH stability: 2-13
Applications: Desalting, peptide separation, biological-extract cleanup, and molecular-weight determination

Features

Fine-particle separation
1500-30000 Da range
Suitable for peptide purification
Extract-cleanup capability
Molecular-weight determination
Controlled dextran pores
Broad CIP pH stability
Improved chromatographic efficiency

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