CM Seplife BB Weak Cation-Exchange Medium, 1200-1800 cm/h (CAT#: STEM-BC-3169-ZJF)

This weak cation-exchange medium is designed for purification of proteins, nucleic acids, and peptides. It uses six-percent crosslinked agarose functionalized with carboxymethyl groups. The weak-acid ligand provides pH-dependent selectivity for positively charged biomolecules. Large spherical particles create a highly permeable bed with low operating backpressure. The medium supports flow from 1200 to 1800 cm/h under the specified pressure condition. Broad chemical stability permits use with common buffers, sodium hydroxide, denaturants, and ethanol. The agarose matrix withstands the stated steam-treatment conditions. Its large-particle format supports rapid processing and straightforward scale-up.

Model: CM Seplife BB
Appearance: White spherical gel, odorless and tasteless
Type: Weak cation-exchange medium
Matrix: 6% crosslinked agarose
Ligand: Carboxymethyl
Median particle size d50: 200 µm
Particle-size range: 100-300 µm
Ion-exchange capacity: 0.09-0.13 mmol/mL
Flow range: 1200-1800 cm/h at 0.1 MPa differential pressure and 25 cm bed height
Operating temperature: 4-30°C
Long-term pH stability: 4-13
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol
Heat resistance: 121°C in water for 30 min
Storage: 20% ethanol at 4-30°C

Ultra-high linear flow
Large permeable particles
Weak carboxymethyl exchange
Low operating backpressure
Broad chemical resistance
Steam-treatment tolerance
Wide CIP pH range
Suitable for large-scale processing

STEM-BC-0412-ZJF
STEM-BC-3074-ZJF
STEM-BC-3075-ZJF
STEM-BC-3078-ZJF
STEM-BC-3081-ZJF