This dextran size-exclusion medium is designed for desalting and separation of peptides and other small molecules. It consists of dextran crosslinked with epichlorohydrin to form a three-dimensional porous network. Molecules are separated according to their ability to enter the internal gel pores. Large molecules are excluded from the pores and therefore elute first. Intermediate-size molecules enter part of the pore volume and elute later. Small molecules access more of the pore network and experience the greatest retention. The fine particle range supports efficient chromatographic separation. The medium provides a stated molecular-weight separation range of 1000-5000 Da.
Specification
Model: Seplife G25 F Appearance: White spherical particles, odorless and tasteless Dry particle size: 20-80 µm Wet particle size: 40-140 µm Swelling volume: 4-6 mL/g dry powder Pressure resistance: 0.24 MPa Maximum flow rate: 480 cm/h pH stability: 2-13 Fractionation range: 1000-5000 Da Chemical compatibility: 6 M guanidine hydrochloride, 8 M urea, 24% ethanol, 0.2 M sodium hydroxide, 0.2 M hydrochloric acid, 1 M acetic acid, 1% SDS, 30% isopropanol, and 30% acetonitrile
Features
Fine-particle dextran medium 1000-5000 Da range Suitable for desalting Up to 480 cm/h flow Broad chemical compatibility Stable crosslinked structure Predictable swelling performance Suitable for peptides