This reversed-phase medium is designed for high-resolution separation of small molecules, peptides, and low-molecular-weight proteins. It uses a porous polystyrene-divinylbenzene matrix. The 15 µm particles provide efficient mass transfer and improved chromatographic resolution. A 100 Å average pore size is suited to small molecules and smaller peptides. The polymeric matrix provides a broader pH range than conventional reversed-phase silica. High rigidity permits operation at elevated pressure and fast flow. The medium is compatible with common aqueous buffers and many organic solvents. High dynamic loading supports productive preparative purification. It can be stored in 20% ethanol between uses.
Specification
Model: Seplife® RP LXMS-15(100) Appearance: White spherical particles Matrix: Polystyrene-divinylbenzene Particle size: 15±2 µm Average pore size: 100 Å Wet apparent density: 0.80-0.90 g/mL Water content: 50-80% Recommended flow rate: 100-1000 cm/h Long-term pH stability: 2-12 Short-term CIP pH stability: 1-14 Dynamic loading: 2-40 mg vitamin B12/mL and 30-60 mg IN/mL Maximum pressure: 5 MPa Storage: 20% ethanol at 4-30°C Chemical compatibility: Common aqueous buffers, strong acids, alcohols, acetone, sodium hydroxide, TFA solutions, acetonitrile, isopropanol, and tetrahydrofuran
Features
15 µm high-resolution particles 100 Å pore structure Broad pH operating range Up to 5 MPa pressure Strong solvent compatibility High-flow performance Suitable for small molecules CIP-compatible polymer matrix