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Reduced Representation Bisulfite Sequencing (RRBS-seq) (CAT#: STEM-MB-0057-WXH)
Introduction
Reduced representation bisulfite sequencing (RRBS) is an efficient and high-throughput technique for analyzing the genome-wide methylation profiles on a single nucleotide level. It combines restriction enzymes and bisulfite sequencing to enrich for areas of the genome with a high CpG content. Due to the high cost and depth of sequencing to analyze methylation status in the entire genome, Meissner et al. developed this technique in 2005 to reduce the amount of nucleotides required to sequence to 1% of the genome.
Applications
• Study methylomes in cancer genomics
• Study methylation states in development
• Study methylation states in development
Procedure
1.Input genomic DNA
2.Restriction enzyme digest
3.Adapter ligation
4.Bisulfite conversion
5.PCR amplify
6.Sequencing
2.Restriction enzyme digest
3.Adapter ligation
4.Bisulfite conversion
5.PCR amplify
6.Sequencing
Notes
Customer provides tissue, cell, DNA and other samples and related information
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