Recombinant Human FLT3 Ligand Protein, Seafrom (CAT#: STEM-CT-0028-ZJF)

The Recombinant Human FLT3 Ligand Protein manufactured by Seafrom is suitable for stimulating growth and differentiation of hematopoietic cells.
FLT3 Ligand (FLT3L) is a growth factor that regulates the proliferation of early hematopoietic cells. FLT3L binds to the cell surface receptor FLT3, which is a receptor with tyrosine kinase activity. FLT3L itself does not stimulate the proliferation of early hematopoietic cells but acts in synergy with other CSFs (colony-stimulating factors) and interleukins to induce growth and differentiation. Unlike SCF (Stem Cell Factor), FLT3L does not affect mast cells. Multiple subtypes of FLT3 Ligand have been identified. The main biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). This membrane-bound isoform can be proteolytically cleaved to generate biologically active soluble isoforms.
The lyophilized formulation can be stored at 2-8°C for 24 months. For reconstitution, it can be dissolved in injection-grade water, physiological saline, culture medium, or PBS. After dissolution, it should be divided into smaller aliquots and stored at -20°C for 6 months or at -80°C for 12 months, with a final concentration after dilution not lower than 100μg/mL. Avoid repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: FLT3LG, FL, FLT3L, Flt3 ligand
Protein Sequence: DNA sequence encodes human FLT3-Ligand (NP_001450.2) with a His tag at the C-terminus, predicted molecular weight of 18.4 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <0.1 EU/μg
Biological Activity: Cell proliferation assay using human OCI-AML5 cells, specific activity ≥5.0 x 10^5 IU/mg
Purification Method: Chromatographic purification
Appearance: White loose body

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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