Molecular Devices SpectraMax M2/M2e Microplate Readers, Wavelength Range 200–1000 nm, Molecular Devices (CAT#: STEM-SPO-0004-LGZ)

The SpectraMax® M2 and M2e systems from Molecular Devices are multiplexed microplate readers with dual monochromators, dual mode tube ports, and top and bottom reading capabilities (top reading only on M2). Detection methods include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Endpoint, kinetic, spectral and area well scan read types and PathCheck® sensors allow for homogeneous and heterogeneous microplate analysis in one flexible system. The SpectraMax M2 reader can be easily converted and optimized for very low-throughput to medium-high throughput analysis, faster, more accurate results and saving reagents. Additionally, by issuing a single read command, combined absorbance and fluorescence intensity assays can be run.

Condition: certified pre-owned
Absorbance photometric performance
Wavelength selection: Monochromator, tunable in 1 nm increments
Wavelength range: 200–1000 nm
Wavelength bandwidth: ≤ 4.0 nm
Wavelength accuracy: ±2.0 nm
Wavelength repeatability: ±0.2 nm
Photometric range: 0–4.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0–2 OD
Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0–2 OD
Photometric precision: < ±0.003 OD ±1.0%, 0–2 OD
Baseline flatness: < 0.001 OD
Stray light: < 0.05% @ 230 nm

Fluorescence photometric performance
Dual monochromators: 1 nm increment selection
EX Wavelength range: 250–850 nm
EM Wavelength range: M2: 360–850 nm, M2e: 250–850 nm
Wavelength bandwidth (EX, EM): 9 nm
Top-read optimized sensitivity: 5 pM (fluorescein) in 96 wells
Top-read guaranteed* sensitivity: 15 pM (fluorescein) in 96 wells
Bottom-read optimized sensitivity (M2e only): 10 pM (fluorescein) in 96 wells
Bottom-read guaranteed* sensitivity (M2e only): 25 pM (fluorescein) in 96 wells

Time-Resolved Fluorescence (secondary mode)
EX Wavelength range: 250–850 nm
EM Wavelength range: M2: 360–850 nm, M2e: 250–850 nm
Data collection: 50–1450 µsec., 200 µsec. increments
Guaranteed sensitivity*: 7 pM Eu-chelate

Luminescence (secondary mode)
Wavelength range (M2): 360–850 nm
Wavelength range (M2e): 250–850 nm
Guaranteed sensitivity*: 50 fM alkaline phosphatase

General photometric performance
Plate formats: 6, 12, 24, 48, 96, 384 wells
Light source: Xenon flash lamp (1 joule/flash)
Detector: Photomultiplier tube (PMT)
Read time**
96 wells: Abs 18 sec., FI 15 sec.
384 wells: Abs 49 sec., FI 45 sec.
Shaker time: 0 to 999 seconds
Temperature control: Ambient +4°C to 45°C
Temperature uniformity: < 1°C at 37°C set point
Temperature accuracy: ±1°C at 37°C set point

Multiple detection capability
Top and bottom read (M2e only)
Dual Mode Test Tube Port
double monochromator
PathCheck and Well Volume Sensors
Instrument and Software Validation
Robot compatible

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