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Acoustic Focusing Cytometry (CAT#: STEM-CBT-0054-WXH)

Introduction

Acoustic cytometry is a new technology that replaces or partly replaces hydrodynamic focusing of cells or particles in flow cytometry with forces derived from acoustic radiation pressure. The ability to focus cells into a tight line without relying on hydrodynamic forces allows many possibilities outside the scope of conventional flow cytometry. Dilute samples can be processed quickly. <br />Acoustic focusing offers many benefits including:<br />10X faster than traditional flow cytometers<br />Broadens experimental capabilities by completing time-consuming experiments such as cell cycle analysis faster and without compromising data quality<br />Accommodates a broader range cell types, from small platelets to large cardiomyocytes




Principle

The acoustic technology generates ultrasonic waves that act to transport particles to the center of the sample stream (acoustic focusing). The pre-focused stream is injected into the sheath stream, which applies hydrodynamic pressure to the sample (traditional hydrodynamic focusing). The combination of these two forces results in a narrow core stream and an increased probability that cells are passing single file through optical system.

Applications

• Capable of performing a full range of cellular analysis including cell cycle, apoptosis, cell concentration, absolute cell count, immunophenotyping, cytokine determination, antibody determination, and cell activity identification.
• Fluorescent protein detection.
• Rare cell analysis.

Procedure

1.Single cell suspension
2.Stain
3.Dilute
4.Analyse by acoustic focusing cytometer

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