LRRK2 Signaling Pathway Assay (CAT#: STEM-MB-0333-WXH)

LRRK2 is closely related to ERK1/2 and Wnt signaling pathways. Both LRRK2 and these two pathways affect the function in the nucleus. Outside the nucleus, the increased protein translation of LRRK2 by inhibiting the protein 4E-BP1 and the increase of mitochondrial autophagy all lead to the state of autophagy/lysosomal stress. LRRK2 promotes the increase of intracellular calcium and activates ERK1/2-dependent autophagy and down-regulation of dendritic mitochondria. LRRK2 and ERK1/2 also regulate the dynamics of microtubules in cells through phosphorylation of tau, actin, and tubulin. LRRK2 also triggers Wnt-β-catenin and ERK1/2-dependent gene transcription changes. The autophagy, transcription, and cytoskeleton effects of mutant LRRK2 expression contribute to ERK1/2-dependent neuron process shortening, while altered endosome/vesicle dynamics affect synaptic function.

To study the regulation mechanism of LRRK2 signal pathway in disease
To study the effect of each virus on LRRK2 signaling pathway
To study the effects of drugs or therapies on LRRK2 signaling pathways

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Detectable targets: BAK, Bax, PIDD, MYD88, Rac1, TLR4, Bcl-xl, RIP1, TRAF-2, FADD, APAF-1, BID, FADD, IRF7, Mda-5, NFκB, RIG-1, TRAF3, GAS, IRF9, MEKK1, p38, RIP1, TRAF5, Histone-H3, IRF5, MEK3, p38MAPK, SH2, TRAF6, IKK-α, IRS1, MEK6, p50, SLP76, TRAM, IKK-β, IRS2, MSK1, p65, Tak1, TRIF, IPS-1, ISGF3, MSK2, PI3K, TBK1, Vav, IRAK1, ISRE, mTOR, PKR, TLR3, p53, BID, IAPs, tBID, SODD, Bad