IL-35 Detection (CAT#: STEM-MB-0301-WXH)

There are four receptors for IL-35, namely IL-12Rβ2: gb130, IL-12Rβ2: IL-12Rβ2, gb130: gb130 and IL-12Rβ2: IL-27Rα. Among them, IL-12Rβ2 is mainly expressed by activated T cells and NK cells, and almost all cells can express gb130. IL-35 binds to IL-12Rβ2: gb130 receptor and first activates the Janus family (Jaks) for signal transduction and activates STAT1 and STAT4 in signal transducers and activators of transcription (STAT). IL-35 can also interact with IL-12Rβ2:IL-12Rβ2 or gb130:gb130 to activate STAT1. IL-12Rβ2 mainly activates JAK1 and JAK2, and phosphorylates downstream STAT4 and STAT1 molecules, respectively. The signal transduction of IL-35 is achieved through the JAK-STAT family.

IL-35 can directly inhibit the tumor immune status of the tumor microenvironment and cause immune escape.
IL-35 can directly act on certain types of tumor cells to promote tumor progression.
IL-35 plays a role in cardiovascular disease, asthma, and autoimmune diseases.

1. Process samples.
2. IL-35 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.