HOOK™ BiotinQuant™ Kit, 20 Assays, BKC-01, G-BIOSCIENCES (CAT#: STEM-GT-3025-LGZ)

HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzobenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-mole ratio of biotin to antibody or protein.

The method for biotin incorporation estimation is based on the binding of avidin to the HABA dye, which produces a color that can be read at 500 nm. The HABA-avidin complex can be replaced by free biotin or biotin conjugated to other molecules (proteins). Measuring the change in optical density of the HABA-avidin complex with a biotinylated protein can accurately estimate the molar ratio of biotin bound to the protein/antibody.

HOOK™ BiotinQuant™ offers premixed aliquots of OneQuant™ HABA/Avidin to eliminate the difficulty of weighing small amounts of reagents and ensure reproducible estimates.

Size: 20 Assays

Accurate Estimation of Biotin Incorporation
Offers OneQuant™ HABA/Avidin to eliminate waste from weighing small batches

Capturem™ His-Tagged Purification 24-Well Plate, 1 x 24-Well Plate, 635730, Takara Bio Inc (CAT#-STEM-GT-3060-LGZ)
Capturem™ His-Tagged Purification 96-Well Plate, 1 x 96-Well Plate, 635714, Takara Bio Inc (CAT#-STEM-GT-3061-LGZ)
TALON® CellThru, 100 mL , 635510, Takara Bio Inc (CAT#-STEM-GT-3062-LGZ)
TALON® CellThru, 10 mL , 635509, Takara Bio Inc (CAT#-STEM-GT-3063-LGZ)
His60 Ni Superflow Resin & xTractor™ Buffer Bundle, 20 Purifications , 635676, Takara Bio Inc (CAT#-STEM-GT-3064-LGZ)
His60 Ni Buffer Set, 20 Purifications , 635665, Takara Bio Inc (CAT#-STEM-GT-3065-LGZ)