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Determination of dissolved inorganic selenium (Se) speciation in environmental and industrial waters by Atomic fluorescence spectrometry (AFS) (CAT#: STEM-ST-0138-WXH)

Introduction

Selenium occurs in both inorganic and organic form. Among the inorganic forms (i.e., selenates, selenides and selenite), the selenide form is more frequently found in the food supply.




Principle

Atomic fluorescence spectroscopy (AFS) is the emission of radiation energy in the UV-visible region from gas-phase atoms that have been excited to higher energy levels by absorption of radiant energy. Usually, a flame is used to obtain the atom in a gaseous state. It is a radiative emission process that proceeds from the lowest singlet (S1) to the singlet ground state(S0).

Applications

Atomic Fluorescence Spectrometry (AFS) is an analytical technique that is primarily used to detect and quantify metals. It is an exceptionally sensitive technique that relies on exploiting the distinctive fluorescent spectra of each specific metal.

Procedure

1. Sample preparetion
2. Sample ionization
3. Detection

Materials

• Line Source
• Nebulizer-burner system
• Monochromators/Filters
• Detector, Amplifier, Readout