Detection of arsenic speciation by Atomic fluorescence spectrometry (AFS) (CAT#: STEM-ST-0139-WXH)

Introduction

Arsenic is a naturally occurring substance that can be found in air, water, and soil. It can also be released into the environment by certain agricultural and industrial processes, such as mining and metal smelting.
Arsenic can cause lung and skin cancers and may cause other cancers. The association between chronic arsenic exposure and cancer is strongest for skin, lung, and bladder cancer. Liver (angiosarcoma), kidney, and other cancers have limited strength of association.

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Principle

Atomic fluorescence spectroscopy (AFS) is the emission of radiation energy in the UV-visible region from gas-phase atoms that have been excited to higher energy levels by absorption of radiant energy. Usually, a flame is used to obtain the atom in a gaseous state. It is a radiative emission process that proceeds from the lowest singlet (S1) to the singlet ground state(S0).

Applications

Atomic Fluorescence Spectrometry (AFS) is an analytical technique that is primarily used to detect and quantify metals. It is an exceptionally sensitive technique that relies on exploiting the distinctive fluorescent spectra of each specific metal.

Procedure

1. Sample preparetion
2. Sample ionization
3. Detection

Materials

• Line Source
• Nebulizer-burner system
• Monochromators/Filters
• Detector, Amplifier, Readout
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