CM Seplife HP Weak Cation-Exchange Medium, 24-44 µm (CAT#: STEM-BC-3167-ZJF)

This weak cation-exchange medium is designed for purification of proteins, nucleic acids, and peptides. It uses highly crosslinked agarose functionalized with carboxymethyl groups. The weak-acid ligand provides pH-dependent binding of positively charged biomolecules. The 24-44 µm particles improve chromatographic resolution and mass transfer. A lysozyme adsorption capacity above 55 mg/mL supports preparative purification. The medium provides a maximum stated flow rate of 150 cm/h. Broad chemical compatibility allows alkaline cleaning and exposure to denaturants and ethanol. The matrix tolerates steam treatment and supports repeated high-resolution purification cycles.

Model: CM Seplife HP
Appearance: White spherical gel, odorless and tasteless
Type: Weak cation-exchange medium
Matrix: Seplife 6FF
Ligand: Carboxymethyl
Particle size: 24-44 µm
Lysozyme adsorption capacity:>55 mg/mL
Ion-exchange capacity: 0.09-0.13 mmol/mL
Maximum flow rate: 150 cm/h
Pressure resistance: 0.3 MPa
Operating temperature: 4-40°C
Long-term pH stability: 4-13
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous buffers, 1 mol/L sodium hydroxide, 8 mol/L urea, 8 mol/L guanidine hydrochloride, and 70% ethanol
Heat resistance: 121°C in water for 30 min

High-resolution 24-44 µm particles
Weak carboxymethyl exchange
>55 mg/mL lysozyme capacity
Broad chemical resistance
Wide CIP pH range
Low nonspecific adsorption
Steam-treatment compatibility
Suitable for fine purification

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