Cationic Polymers method for transient transfection (CAT#: STEM-GT-0005-WXH)

Using cationic polymers, nucleotides can be transiently transfected into eukaryotic cells in an inexpensive and simple manner. After protocol optimization, the results can be easily reproduced. As a drawback, transfection by cationic polymers has a very low efficiency (<10%) in a number of cell types, including primary cells. Further, cationic polymers are highly cytotoxic and therefore not suitable for transfection of sensitive cells and generation of stable cell lines.

Stable transduction

1. Mix nucleic acid with cationic polymer solution in transfection medium or phosphate-buffered saline solution.
2. Add the nucleic acid-cationic polymer complexes to the cells.
3. Assay cells for transient gene expression.