Electroporation (CAT#: STEM-GT-0008-WXH)

During electroporation, a mixture of the cells and the nucleotide of interest is exposed to an intense electric field. This leads to transient cell membrane destabilization, making the cell membrane permeable to the nucleotides that are present in the surrounding solution. The cell membrane is transiently permeabilized by a short electric pulse, allowing the nucleotides, which are present in the surrounding solution, to enter the cytoplasm. After removing the electric field, the cell membrane stabilizes, enclosing the nucleotides in the cytoplasm, where they are expressed.

Transient and stable transfection

1. Prepare cells by suspending in electroporation buffer.
2. Apply electrical pulse to cells in the presence of specialized buffer and nucleic acids.
3. Return cells to growth conditions and allow them to recover.
4. Assay cells for gene expression or silencing.