Calcium phosphate transfection (CAT#: STEM-GT-0004-WXH)

Introduction

The calcium phosphate transfection is an inexpensive and simple method for transient or stable nucleotide transfer into most cell lines. The transfection efficiency, however, strongly depends on the cell constitution, the pH, and the quality and the amount of the used nucleotides. Therefore, the optimal experimental conditions have to be tediously established in advance. Further, the calcium phosphate transfection is toxic and therefore not suitable for most sensitive primary cell lines.