Bacterial Two-hybrid (B2H) System to study protein-protein interactions (CAT#: STEM-MB-0019-WXH)

Introduction

Protein-protein interaction(PPI) plays an essential role in virtually every cellular process and the identification of it is a key step to understand unknown and complex protein functions. <br />The bacterial two-hybrid is a rapid genetic approach to detect and characterize interactions between a wide variety of bacterial, eukaryotic, or viral proteins in vivo. Similarly to the classic yeast two-hybrid (Y2H), bacterial two-hybrid is based on reconstituting the activity of a certain protein from its separate domains and a reporter gene is expressed only when the relative protein-protein interaction occurs within a test cell. It is worth noting that the phenomenon of autoactivation by baits is a less problem in bacterial systems than in the yeast. Therefore, the bacterial method provides an alternative strategy for studying proteins which cannot be assayed in Y2H because of toxicity or low expression.