Ribosome display technology to screen proteins (CAT#: STEM-MB-0027-WXH)

Introduction

Ribosome display technology is a powerful tool for screening proteins. Under certain conditions, mRNA and protein are bound to ribosomes at the same time to form a "protein-ribosome-mRNA" (PRM) ternary complex in the form of non-covalent bonds. By linking genotype and protein phenotype in Together, the target protein and the corresponding gene sequence are screened from the protein display library using the specific ligand of the target protein.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Screening of antibodies
• Screening for non-antibody proteins
• Enzyme affinity analysis
• Peptide Screening and Affinity Analysis
• Identification of antigenic determinants
• Molecular evolution

Procedure

1.Construction of templates
2.In vitro transcription and in vitro translation
3.Affinity screening
4.In vitro molecular directed evolution

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