Yeast Display Library Construction for protein expression (CAT#: STEM-MB-0033-WXH)

Introduction

Yeast surface display technology is a eukaryotic protein expression system that uses a yeast cell surface display system (with well-established protein post-translational modification and secretion mechanisms) to immobilize and display heterologous eukaryotic proteins on the surface of yeast cells, and has been used in protein library screening, protein directed evolution, high-affinity antibody sorting, antigen/antibody library construction, affinity maturation, vaccine production, immunobiocatalysis and biosensors, among many other applications. The basic principle is to fuse an exogenous target gene with a specific vector gene sequence and introduce it into yeast cells, and use the yeast intracellular protein transport mechanism to transfer the target protein to the cell surface and anchor it to the cell membrane.<br />Compared with the traditional phage display technology, the yeast surface display system is a new method for screening peptides and proteins with specific properties by combining random mutagenesis and combinatorial library, which can avoid the non-specific binding of phage itself to target proteins and it is an important complement to the phage surface display system.