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Assessing the Hydrolysis of Biopharmaceutical Compounds by Liquid-Chromatography Single Quad Mass Detector (LC-QDa) (CAT#: STEM-B-0410-CJ)

Introduction

All biopharmaceutical compounds are sensitive towards chemical degradation. Examples are deamidation, hydrolysis, oxidation, photo-degradation, disulfide-scrambling, and others. Chemical degradation can lead to aggregation, charge variants and/or structural changes of the drug substance and eventually impair the effectivity or safety of the therapy.




Principle

Liquid-chromatography single quad mass detector (LC-QDa) is the development of a LC/MS method for quantifying free fatty acids in both PS20 and PS80-containing biopharmaceutical formulations by using a single quad mass detector (QDa), which can be readily integrated into a routine QC environment.

QDA, because it allows for more flexibility for the covariance matrix, tends to fit the data better than LDA, but then it has more parameters to estimate. The number of parameters increases significantly with QDA. Because, with QDA, you will have a separate covariance matrix for every class.

Applications

Biopharmaceutica

Procedure

1. Prepare samples and reagents
2. Connect the LC to the QDa detector.
3. Set the experiment according to the instructions and analyze the data.

Materials

• Sample: Peptides, Proteins, Vaccines, Virus-like particles, Antibody
• Equipment: QDa Mass Detector