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Antibody Purification by Protein A Magnetic Beads (CAT#: STEM-MB-1306-LGZ)
Introduction
Magnetic bead purification technology has significant advantages over traditional protein and nucleic acid purification techniques. Rapid purification of proteins and antibodies is simple with coated magnetic beads. Save time and money by replacing nucleic acid-based purification columns with silica-coated magnetic bead technology.
Principle
Antibodies easily bind to the Protein A Magnetic Beads due to their high affinity for protein A. The target protein is captured by the antibody:Protein A Magnetic Beads and are temporarily immobilized at the tube wall by magnetic attraction and all other proteins are easily removed.
Applications
Protein A Magnetic Beads are designed as a rapid and simple tool for immunoprecipitation, purification and/or depletion assays, and other magnetic separation applications.
Procedure
1. Buffer preparation.
2. Sample Preparation
3. Bead preparation.
4. Purification of target protein.
2. Sample Preparation
3. Bead preparation.
4. Purification of target protein.
Materials
• Washing Buffer
• Elution Buffer
• Neutralization Buffer
• Storage Buffer
• Magnetic Stand or magnet
• Elution Buffer
• Neutralization Buffer
• Storage Buffer
• Magnetic Stand or magnet
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