Analysis of Apoptosis by Flow Cytometry (CAT#: STEM-CBT-0060-WXH)

Introduction

Apoptosis, or programed cell death, is a phenomenon that is frequently examined in immunology and other fields of study. It is used by the immune system to maintain the homeostasis by removing cells without triggering an inflammatory response. This is in contrast to necrosis, a type of cell death that does trigger an inflammatory response. Apoptosis is the mechanism of cell death for clonally expanded T cells following an immune response, for self-targeting T cells, for autoreactive B cells, and multiple other cells in the immune system.<br />Flow cytometry is widely used to analyze apoptosis due to the versatility afforded by its several technological benefits:<br />Multiparameter analytical capabilities – Ability to analyze several parameters of apoptosis in a single assay<br />High-throughput analysis of large numbers of cells<br />Quantitative data gathering on percentages, as well as absolute number of cells<br />Single cell resolution – individual cell-based mode of analysis