Treponema erosive Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1415-ZJF)

This real-time PCR detection kit is based on probe technology and is designed to identify Treponema erosive through specific amplification of conserved gene regions. Fluorescent signals generated during the PCR process are detected in real time, enabling precise qualitative and quantitative assessment of Treponema erosive DNA in various sample types.

Kit Components:
Treponema erosive Amplification Mix: 50 µL × 1 tube
Treponema erosive Reaction Mix: 950 µL × 1 tube
Treponema erosive Positive Control: 50 µL × 1 tube
Treponema erosive Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Simplified operation with pre-mixed reagents requiring only DNA input.
Uses conserved region alignment to ensure high specificity.
Detects low-copy targets with high sensitivity due to optimized chemistry.
Contains a positive control for result validation.
Enclosed one-tube protocol limits contamination.
Fast turnaround for time-sensitive applications.
Restricted to laboratory research applications.

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