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Structural analysis of Chlorophyll by Circular Dichroism (CD) (CAT#: STEM-MB-0623-WXH)
Introduction
Chlorophyll is the name of the green pigment that plants use to make food during a process called photosynthesis. But if you try to buy it as a supplement, you will likely see it called chlorophyllin, which is a water-soluble form of chlorophyll that contains copper and sodium.
Principle
Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.
Applications
Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.
Procedure
1. Sample preparation
2. Measurement by CD instrument
3. Data analysis
2. Measurement by CD instrument
3. Data analysis
Materials
Circular dichroism (CD) spectrophotometer
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