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Characterization of Membrane-Active Peptides by Circular dichroism (CD) (CAT#: STEM-MB-0641-WXH)
Introduction
Peptides that interact with the cell membrane by disrupting it, by passing through it, or by residing at the membrane interface and fusing with it are known as membrane active peptides.
Principle
Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.
Applications
Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.
Procedure
1. Sample preparation
2. Measurement by CD instrument
3. Data analysis
2. Measurement by CD instrument
3. Data analysis
Materials
Circular dichroism (CD) spectrophotometer
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