SP Seplife C50 Strong Cation-Exchange Medium, 100 mg Ribonuclease/mL (CAT#: STEM-BC-3170-ZJF)

This strong cation-exchange medium is designed for separation of low-molecular-weight proteins, peptides, nucleotides, and selected macromolecules. It uses a highly crosslinked dextran G50 matrix. Sulfopropyl groups provide strong cation-exchange functionality. The dry particles range from 40 to 120 µm and swell to form the working chromatographic bed. A ribonuclease adsorption capacity of 100 mg/mL supports preparative separation. The medium provides flow above 100 cm/h with relatively low backpressure. It is stable in common aqueous buffers and concentrated urea or guanidine hydrochloride. The matrix tolerates the stated steam treatment in sodium chloride solution.

Model: SP Seplife C50
Appearance: White particles, odorless and tasteless
Type: Strong cation-exchange medium
Matrix: Dextran G50
Ligand: Sulfopropyl
Dry particle size: 40-120 µm
Maximum flow rate:>100 cm/h
Ribonuclease adsorption capacity: 100 mg/mL
Ion-exchange capacity: 2.0-2.5 mmol/g dry gel
Long-term pH stability: 2-13
Short-term pH stability: 2-13
Chemical compatibility: Common aqueous buffers, 8 M urea, and 6 M guanidine hydrochloride
Heat resistance: 121°C in 0.1 M sodium chloride for 30 min
Applications: Low-molecular-weight proteins, peptides, nucleotides, and macromolecule separation

Strong sulfopropyl exchange
100 mg/mL ribonuclease capacity
Crosslinked dextran matrix
Suitable for small biomolecules
More than 100 cm/h flow
Denaturant-compatible operation
Steam-treatment tolerance
Scalable preparative separation

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