Simultaneous detection of pH changes and histamine release from oxyntic glands in isolated stomach by amperometry (CAT#: STEM-PPA-0020-LJX)

Amperometric titration is an electrode titration chemical analysis method that indicates the PH of the solution according to the current change of the solution in the electrolytic cell. The theory is based on the combination of two metals can form an electrolytic cell to provide current. Since hydrogen ions are involved in the reaction, the current of the cell indicates the pH value of the solution.

For determining the approximate PH value of solutions in food production, wastewater treatment, pharmaceutical and chemical industries

1. Select the "pH" file of the instrument, immerse the cleaned electrode into the standard pH buffer solution to be measured, press the measurement button, turn the positioning adjustment knob, so that the pH value displayed by the instrument is stable at the pH value of the standard buffer solution
2. Release the measuring button, take out the electrode, rinse with distilled water several times, and carefully blot the solution on the electrode with filter paper
3. Place the electrode in the liquid to be tested, press the measurement button, read the stable pH value, and record
4. Release the measurement button, remove the electrode, clean according to step 2, and continue to measure the next sample solution
5. After measuring, clean the electrode and soak the glass electrode in distilled water

• Sample Type:
Guinea pig gastric juices

When measuring pH value, it is necessary to ensure that the bulb of the electrode is completely immersed in the measured medium, so as to obtain more accurate measurement results.