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Determining the pH of glucose and urea by amperometry (CAT#: STEM-PPA-0016-LJX)

Introduction

A facile and effective electrochemical sensing platform for the detection of glucose and urea in one sample without separation was developed using chitosan-reduced graphene oxide (CS-rGO)/concanavalin A (Con A) as a sensing layer. The CS-rGO/Con A with pH-dependent surface net charges exhibited pH-switchable response to negatively charged Fe(CN)6(3-). The principle for glucose and urea detection was essentially based on in situ pH-switchable enzyme-catalyzed reaction in which the oxidation of glucose catalyzed by glucose oxidase or the hydrolyzation of urea catalyzed by urease resulted in a pH change of electrolyte solution to give different electrochemical responses toward Fe(CN)6(3-).




Principle

Amperometric titration is an electrode titration chemical analysis method that indicates the PH of the solution according to the current change of the solution in the electrolytic cell. The theory is based on the combination of two metals can form an electrolytic cell to provide current. Since hydrogen ions are involved in the reaction, the current of the cell indicates the pH value of the solution.

Applications

For determining the approximate PH value of solutions in food production, wastewater treatment, pharmaceutical and chemical industries

Procedure

1. Select the "pH" file of the instrument, immerse the cleaned electrode into the standard pH buffer solution to be measured, press the measurement button, turn the positioning adjustment knob, so that the pH value displayed by the instrument is stable at the pH value of the standard buffer solution
2. Release the measuring button, take out the electrode, rinse with distilled water several times, and carefully blot the solution on the electrode with filter paper
3. Place the electrode in the liquid to be tested, press the measurement button, read the stable pH value, and record
4. Release the measurement button, remove the electrode, clean according to step 2, and continue to measure the next sample solution
5. After measuring, clean the electrode and soak the glass electrode in distilled water

Materials

• Sample Type:
Glucose and urea

Notes

When measuring pH value, it is necessary to ensure that the bulb of the electrode is completely immersed in the measured medium, so as to obtain more accurate measurement results.