Seplife Oligo dT(20) Affinity Medium, 50 µm (CAT#: STEM-BC-3127-ZJF)

This affinity chromatography medium is designed for selective capture of messenger RNA from complex mixtures. It uses a highly crosslinked polystyrene-divinylbenzene matrix with a hydrophilic surface treatment. Covalently attached Oligo dT(20) groups bind specifically to Poly(A) tails through base pairing. The hydrophilic surface minimizes nonspecific interactions with other sample components. Uniform 50 µm particles support consistent packed-bed performance and rapid mass transfer. The rigid matrix provides high salt tolerance, pressure resistance, and flow above 400 cm/h. Captured mRNA can be released under standard purification conditions. The medium is chemically stable in concentrated salt, selected alcohols, acetonitrile, and isopropanol solutions.

Model: Seplife® Oligo dT (20)
Appearance: White spherical particles
Type: Affinity chromatography medium with dT(20) functionality
Matrix: Polystyrene-divinylbenzene
Ligand: Oligo dT(20)
Ligand density: 0.25-0.35 µmol dT/mL
Average particle size: 50 µm
pH stability: 2-13
Flow rate:>400 cm/h at 0.3 MPa with 0.1 M sodium chloride
Recommended operating temperature: 2-65°C
Chemical compatibility: 5 M sodium chloride, 20% and 70% ethanol, 20% acetonitrile, and 30% isopropanol
Recommended CIP: 0.1 M sodium hydroxide
Storage solution: 20% ethanol

Specific Poly(A) mRNA capture
Covalently attached dT(20)
Uniform 50 µm particles
High salt tolerance
Low nonspecific adsorption
High-flow rigid matrix
Wide operating-temperature range
Alkali-cleanable medium

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