This dextran size-exclusion medium is designed for preparative desalting and purification of peptides and small molecules. It is formed by crosslinking dextran with epichlorohydrin through ether bonds. The resulting three-dimensional gel network separates molecules according to size. Large molecules cannot enter the pores and therefore move through the bed rapidly. Intermediate molecules enter part of the internal pore volume. Small molecules penetrate more of the matrix and elute later. The medium particle size supports preparative column operation. Its stated molecular-weight separation range is 1000-5000 Da.
Specification
Model: Seplife G25 M Appearance: White spherical particles, odorless and tasteless Dry particle size: 50-150 µm Wet particle size: 70-250 µm Swelling volume: 4-6 mL/g dry powder Pressure resistance: 0.24 MPa Maximum flow rate: 480 cm/h pH stability: 2-13 Fractionation range: 1000-5000 Da Chemical compatibility: 6 M guanidine hydrochloride, 8 M urea, 70% ethanol, 0.2 M sodium hydroxide, 0.2 M hydrochloric acid, 1 M acetic acid, 1% SDS, 30% isopropanol, and 30% acetonitrile
Features
Preparative particle size 1000-5000 Da fractionation Suitable for desalting Broad chemical resistance Up to 480 cm/h flow Stable crosslinked dextran Predictable swelling volume Suitable for peptide purification