Seplife G25 M Dextran Size-Exclusion Medium, 50-150 µm (CAT#: STEM-BC-3183-ZJF)

Highlights

Preparative particle size
1000-5000 Da fractionation
Suitable for desalting

Cat Number: STEM-BC-3183-ZJF

Application: for preparative desalting and peptide separation

Model: Seplife G25 M




Description

This dextran size-exclusion medium is designed for preparative desalting and purification of peptides and small molecules. It is formed by crosslinking dextran with epichlorohydrin through ether bonds. The resulting three-dimensional gel network separates molecules according to size. Large molecules cannot enter the pores and therefore move through the bed rapidly. Intermediate molecules enter part of the internal pore volume. Small molecules penetrate more of the matrix and elute later. The medium particle size supports preparative column operation. Its stated molecular-weight separation range is 1000-5000 Da.

Specification

Model: Seplife G25 M
Appearance: White spherical particles, odorless and tasteless
Dry particle size: 50-150 µm
Wet particle size: 70-250 µm
Swelling volume: 4-6 mL/g dry powder
Pressure resistance: 0.24 MPa
Maximum flow rate: 480 cm/h
pH stability: 2-13
Fractionation range: 1000-5000 Da
Chemical compatibility: 6 M guanidine hydrochloride, 8 M urea, 70% ethanol, 0.2 M sodium hydroxide, 0.2 M hydrochloric acid, 1 M acetic acid, 1% SDS, 30% isopropanol, and 30% acetonitrile

Features

Preparative particle size
1000-5000 Da fractionation
Suitable for desalting
Broad chemical resistance
Up to 480 cm/h flow
Stable crosslinked dextran
Predictable swelling volume
Suitable for peptide purification

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