Seplife G25 C Dextran Size-Exclusion Medium, 1000-5000 Da (CAT#: STEM-BC-3087-ZJF)

This dextran chromatography medium is designed for desalting and separation of peptides and other small molecules. It consists of crosslinked dextran with a three-dimensional porous network. Molecular separation occurs according to the ability of solutes to enter the gel pores. Molecules larger than the pore structure are excluded and elute first. Intermediate-size molecules enter part of the pore volume and elute later. Small molecules enter more of the internal pore network and experience the greatest delay. The stated fractionation range is 1000 to 5000 Da. The dry particles swell in liquid to form the working gel bed. Broad solvent compatibility supports use with denaturants, alcohols, acids, bases, detergents, and acetonitrile.

Model: Seplife G25 C
Appearance: White spherical particles, odorless and tasteless
Type: Crosslinked dextran size-exclusion medium
Dry particle size: 100-300 µm
Wet particle size: 180-500 µm
Swelling volume: 4-6 mL/g dry powder
Pressure resistance: 0.24 MPa
Maximum flow rate: 480 cm/h
pH stability: 2-13
Fractionation range: 1000-5000 Da
Chemical compatibility: 6 M guanidine hydrochloride, 8 M urea, 70% ethanol, 0.2 M sodium hydroxide, 0.2 M hydrochloric acid, 1 M acetic acid, 1% SDS, 30% isopropanol, and 30% acetonitrile

Size-exclusion separation mechanism
1000-5000 Da fractionation range
Suitable for desalting
Up to 480 cm/h flow
Broad solvent compatibility
Stable crosslinked dextran matrix
Predictable swelling behavior
Suitable for peptides and small molecules

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