Seplife CL-4B Crosslinked Size-Exclusion Medium, 60000-20×10⁶ Da (CAT#: STEM-BC-3179-ZJF)

This crosslinked agarose medium is designed for size-based purification of proteins, nucleic acids, and peptides. It uses four-percent crosslinked agarose gel particles. Molecular separation is based on differential access to the internal pore network. The very broad exclusion range supports purification of large biomolecules and assemblies. Low nonspecific adsorption helps maintain product recovery. The crosslinked structure provides resistance to denaturants and multiple organic solvents. Broad pH stability supports repeated cleaning-in-place. The particles can be reused for multiple purification cycles. The medium is suited to samples with large molecular-size differences and moderate resolution requirements.

Model: Seplife CL-4B
Appearance: White spherical gel, odorless and tasteless
Matrix: 4% crosslinked agarose gel
Globular-protein exclusion range: 60000-20×10⁶ Da
Particle size: 45-165 µm
Pressure-flow range: 80-150 cm/h at 0.15 MPa
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 8 mol/L urea, 6 mol/L guanidine hydrochloride, ethanol, DMF, THF, DMS, chloroform, acetone, pyridine, dimethylformamide, dichloroethane, and acetonitrile
Heat resistance: 120°C in pH 7 water for 30 min

Very broad exclusion range
Organic-solvent compatibility
Wide CIP pH tolerance
Crosslinked agarose structure
Low nonspecific adsorption
Reusable particles
Suitable for large biomolecules
Stable gel-filtration performance

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