This dextran size-exclusion medium is designed for desalting, peptide separation, biological-extract cleanup, and molecular-weight measurement. It uses an epichlorohydrin-crosslinked dextran matrix with a porous three-dimensional network. Molecular separation is governed by access to the internal pore volume. Large molecules are excluded from the gel and elute before smaller components. Intermediate molecules enter some pores and show moderate retention. Small molecules penetrate more of the matrix and elute later. The medium particle range supports general preparative chromatography. The stated fractionation range is 1500-30000 Da.
Specification
Model: Seplife G50 M Appearance: White spherical particles, odorless and tasteless Dry particle size: 50-150 µm Swelling volume: 9-11 mL/g dry powder Fractionation range: 1500-30000 Da Pressure behavior: Follows Darcy's law Flow behavior: Follows Darcy's law Long-term pH stability: 2-10 Short-term CIP pH stability: 2-13 Applications: Desalting, peptide separation, biological-extract cleanup, and molecular-weight determination
Features
General preparative grade 1500-30000 Da range Suitable for desalting Peptide fractionation Extract-cleanup application Stable dextran network Broad short-term pH range Molecular-weight measurement