Seplife 6FF High-Flow Size-Exclusion Medium, 10000-4×10⁶ Da (CAT#: STEM-BC-3180-ZJF)

This high-flow agarose medium is designed for gel-filtration purification of proteins, nucleic acids, and peptides. It is produced by crosslinking a six-percent agarose base matrix. The enhanced crosslinking improves chemical stability and mechanical strength. Molecules are separated according to their hydrodynamic size and pore accessibility. The medium provides a globular-protein exclusion range from 10000 to 4×10⁶ Da. High flow and relatively low backpressure support efficient preparative processing. Low nonspecific adsorption and high recovery support repeated downstream use. Broad chemical compatibility permits alkaline cleaning and exposure to alcohols, acetonitrile, detergents, and denaturants. The matrix also tolerates the stated steam-treatment conditions.

Model: Seplife 6FF
Appearance: White spherical gel, odorless and tasteless
Matrix: 6% crosslinked agarose gel
Globular-protein exclusion range: 10000-4×10⁶ Da
Particle size: 45-165 µm
Flow rate: 300 cm/h
Pressure resistance: 0.2 MPa
Long-term pH stability: 2-12
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous solutions, 2 M sodium hydroxide, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6 M guanidine hydrochloride, and 8 M urea
Heat resistance: 121°C in pH 7 water for 30 min

High 300 cm/h flow
Crosslinked 6% agarose
Broad chemical compatibility
Wide CIP pH range
Low nonspecific adsorption
High product recovery
Steam-treatment tolerance
Reusable downstream medium

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