Seplife 4FF High-Flow Size-Exclusion Medium, 60000-20×10⁶ Da (CAT#: STEM-BC-3176-ZJF)

This high-flow agarose medium is designed for gel-filtration purification of proteins, nucleic acids, and peptides. It is produced by further crosslinking a four-percent agarose base matrix. The enhanced crosslinking improves chemical stability and mechanical performance. Molecules are separated according to their hydrodynamic size and access to the internal pores. The broad exclusion range supports processing of large biological molecules. The medium provides relatively high flow and low nonspecific adsorption. High product recovery and repeatable reuse support preparative downstream processing. Broad pH and solvent compatibility permit alkaline cleaning and exposure to denaturants. The matrix also tolerates the stated steam-treatment conditions.

Model: Seplife 4FF
Source specification product grade: Seplife 4B
Appearance: White spherical gel, odorless and tasteless
Matrix: 4% crosslinked agarose gel
Globular-protein exclusion range: 60000-20×10⁶ Da
Particle size: 45-165 µm
Flow rate: 250 cm/h at 0.1 MPa
Pressure resistance: 0.15 MPa
Long-term pH stability: 2-12
Short-term CIP pH stability: 2-14
Chemical compatibility: Common aqueous solutions, 2 M sodium hydroxide, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6 M guanidine hydrochloride, and 8 M urea
Heat resistance: 121°C in pH 7 water for 30 min

High-flow agarose matrix
Broad exclusion range
Strong chemical stability
Wide CIP pH tolerance
Low nonspecific adsorption
High product recovery
Steam-treatment compatibility
Reusable process medium

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