rProtein A Seplife Suno Antibody Affinity Medium, 70 mg/mL (CAT#: STEM-BC-3084-ZJF)

This Protein A affinity medium is designed for capture and purification of monoclonal and polyclonal antibodies. It uses highly crosslinked spherical agarose microspheres as the base matrix. An alkali-resistant recombinant Protein A ligand is immobilized on the matrix. The ligand selectively binds the Fc region of antibodies. One affinity step can recover high-purity antibodies from ascites, serum, or cell-culture fluid. A stated binding capacity of 70 mg/mL supports productive capture operations. The medium provides flow above 250 cm/h under the specified pressure condition. Resistance to sodium hydroxide supports repeated cleaning and reuse. Comparative testing showed antibody purity, recovery, and capacity close to the referenced benchmark medium.

Model: rProtein A Seplife Suno
Appearance: Spherical gel, odorless and tasteless
Matrix: Highly crosslinked agarose microspheres
Ligand: Alkali-resistant recombinant Protein A
Flow rate:>250 cm/h at 0.1 MPa
Pressure resistance: 0.3 MPa
Particle size: 65 µm
Binding capacity: 70 mg/mL
Ligand density: 8-9 mg/mL
Alkali resistance: 0.1-0.5 M sodium hydroxide
Example purification result: 54.6 mg/mL loading, 96.9% SEC purity, and 99.2% recovery
CIP example: 0.5 M sodium hydroxide

Specific antibody Fc binding
High 70 mg/mL capacity
Alkali-resistant Protein A ligand
High-flow agarose matrix
One-step antibody capture
Suitable for serum and culture fluid
Reusable after alkaline cleaning
High recovery and purity

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