Red Seplife FF Dye-Affinity Medium, ≥3 mg LDH/mL (CAT#: STEM-BC-3149-ZJF)

This dye-affinity chromatography medium is designed for purification of selected enzymes and plasma proteins. It uses a high-flow agarose matrix coupled with Reactive Red 120. The multiring dye has structural similarities to naturally occurring NADP+. It can bind strongly to proteins requiring nucleotide cofactors. Lactate dehydrogenase is a representative target for specific binding. Albumin, lipoproteins, coagulation factors, and interferons can also interact through electrostatic or hydrophobic mechanisms. High-flow particles support rapid preparative processing. The medium tolerates selected denaturants and ethanol under the stated conditions.

Model: Red Seplife FF
Appearance: White spherical particles, odorless and tasteless
Matrix: Seplife 6FF
Ligand: Reactive Red 120
Particle size: 45-165 µm
Maximum flow rate: 750 cm/h
Ligand density: 2 µmol/mL
Binding capacity: ≥3 mg lactate dehydrogenase/mL
Long-term pH stability: 3-13
Short-term CIP pH stability: 4-12
Chemical compatibility: 6 M guanidine hydrochloride, 8 M urea, and 70% ethanol
Applications: Albumin, interferons, coagulation factors, and NAD+/NADP+-dependent enzymes

Reactive Red 120 ligand
≥3 mg/mL LDH capacity
Up to 750 cm/h flow
Suitable for cofactor-dependent enzymes
Multiple binding mechanisms
Denaturant-compatible matrix
Broad application range
High-flow agarose support

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