Recombinant Human IL-1α Protein, Seafrom (CAT#: STEM-CT-0012-ZJF)

The Recombinant Human IL-1α Protein manufactured by Seafrom is suitable for inflammation modulation, immune response, and cellular processes.
Interleukin-1 (IL-1) is a peptide with a molecular weight of 17 kD and consists of two subtypes, IL-1α and IL-1β. IL-1α is composed of 159 amino acids, while IL-1β contains 153 amino acids. They are encoded by different genes. Despite having only 26% homology in their amino acid sequences, IL-1α and IL-1β bind with the same affinity to the same cell surface receptor and exert the same biological functions. IL-1 receptors (IL-1R) are present on the surface of nearly all nucleated cells, with the number of IL-1R varying from cell to cell, ranging from a few dozen (e.g., T cells) to several thousand (e.g., fibroblasts). IL-1R primarily has two types: IL-1r1, which has a longer intracellular peptide chain, plays a role in transmitting activation signals; and IL-1R2, which has a shorter intracellular peptide chain and cannot effectively transmit signals. Instead, it releases the extracellular peptide chain into the extracellular fluid, where it binds to IL-1 in a free form, exerting a feedback inhibitory effect. GM-CSF, G-CSF, and IL-1 itself can increase the expression of cell IL-1R, while TGF and corticosteroids can reduce IL-1R expression. Suitable for the production of cell therapy products.
Lyophilized preparations can be stored at -20°C. For aliquoting, they can be reconstituted with injection water, physiological saline, culture medium, or PBS. After reconstitution, divide into smaller portions and store at -20°C for 6 months or at -80°C for 12 months. The final concentration after dilution should not be lower than 100μg/mL. Avoid repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: L1A, IL-1α, IL1F1, IL1α
Protein Sequence: DNA sequence encodes human IL-1α (P01583-1: S113-A271) expressed with a C-terminal His tag
Molecular Weight: Predicted theoretical molecular weight of 18.9 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <0.1 EU/μg
Biological Activity: Induction of interferon-γ secretion in NK-92 cells, ED50≤100 ng/mL
Purification Method: Chromatographic purification
Appearance: White loose body

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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