ProteoSure™ Recombinant Bovine Enterokinase, 5 x 1 KU, 14-REK-5KU, Marvelgent biosciences (CAT#: STEM-GT-3037-LGZ)

Enterokinase is a special protease that cleaves at the carboxyl side of a lysine residue when it is preceded by four aspartic acid groups (Asp-Asp-Asp-Asp-Lys) and followed by no proline . Enterokinase can be used to remove the fusion tag of a protein or the N-terminal presequence of a precursor protein that has been genetically engineered to be activated by enterokinase.

ProteoSure™ Recombinant Bovine Enterokinase is produced in E. coli. And it does not contain contaminating proteases. The enzyme is purified by a proprietary process for high purity and maximum enzyme activity. This product is supplied as a clear aqueous solution (≥5 U/μL) in a specially formulated storage buffer to ensure maximum stability of the enzyme during storage.

The enzyme is highly active at 4°C, allowing digestion at ideal low temperatures for maximum stability of temperature-sensitive target substrates during digestion.

Unit definition: 1 unit is defined as the amount of enzyme required to cleave 0.5 mg of DDDDK-containing protein (approximately 30 kDa in size) to 95% within 12 - 16 hours at 25°C.

Size: 5 x 1 KU
Synonyms: Enteropeptidase
CAS #: 9014-74-8
Enzyme Commission #: 3.4.21.9
Molecular weight: Theoretical MW: 25.8 kDa; Apparent MW on SDS-PAGE: ~ 27 kDa
Origin of species: Bovine
Source: E.coli
Appearance: Clear aqueous solution, filter-sterilized
Specific activity: ≥5 U/μL
Unit definition: One unit is defined as the amount of enzyme needed to cleave 0.5 mg of a special fusion protein in 12 to 16 hours to get 95% completion at 25°C in a buffer containing 25 mM Tris-HCl (pH 8.0).
Storage & stability: The product is shipped with blue ice. Store at -20°C upon receipt. It remains stable without detectable loss of activity after one week at 25°C. Stable after several freeze-thaw cycles.
Intended use: For research or further processing only. Not intended for any human or animal diagnostic or therapeutic use.

High activity
One unit is capable of cleaving 0.5 mg of ddddk-containing protein to 95% in 12 to 16 hours at 25°C. Supplied as a concentrated solution of >5 u/µL.

High purity
Free of any other protease contaminants or impurities of animal origin.

High specificity
Protease that cleaves specifically after lysine with four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK)
No off-target cleavage was detected when used at 2 - 25°C.
Long-lasting superior performance. Enzyme activity is maintained at 2 - 8°C.
It allows low-temperature digestion to ensure the stability of temperature-sensitive target proteins

Compatible with the following reagents:
Imidazole (<100 mM), NaCl (<50 mM), and glycerin (<5%).

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