Phenyl Seplife FF HS Hydrophobic Interaction Medium, 40 mg HSA/mL (CAT#: STEM-BC-3133-ZJF)

This hydrophobic interaction chromatography medium is designed for separation of biological macromolecules. It uses a high-flow agarose matrix functionalized with phenyl groups. The strong hydrophobic ligand interacts with exposed hydrophobic regions on proteins and other biomolecules. Samples bind under elevated salt conditions and elute as salt concentration is reduced. Differences in surface hydrophobicity provide the basis for chromatographic separation. The medium offers high protein capacity and rapid linear flow. Spherical particles support uniform bed packing and reproducible purification. Broad pH and chemical stability permit repeated cleaning and regeneration. The medium is suitable for capture, intermediate purification, and polishing of compatible biomolecules.

Model: Phenyl Seplife FF(HS)
Appearance: White spherical gel, odorless and tasteless
Matrix: Seplife 6FF
Ligand: Phenyl
Shape: Spherical
Particle size: 45-165 µm
Maximum flow rate: 600 cm/h
Pressure resistance: 0.3 MPa
Operating temperature: 4-40°C
Ligand density: 45 µmol phenyl/mL medium
Protein adsorption capacity: 40 mg HSA/mL medium
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 1 mol/L sodium hydroxide, 70% ethanol, 30% isopropanol, 0.5% SDS, 10% ethylene glycol, 6 mol/L guanidine hydrochloride, 8 mol/L urea, and 3 mol/L ammonium sulfate

Strong phenyl hydrophobicity
40 mg/mL HSA capacity
Up to 600 cm/h flow
Broad CIP pH range
High-flow agarose matrix
Strong chemical compatibility
Uniform spherical particles
Suitable for biomacromolecule purification

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