Oncorhynchus keta Genomic DNA Residual Probe-Based qPCR Kit (No Internal Control), 50 Tests/Box (CAT#: STEM-SLSR-1691-ZJF)

This kit offers a robust and sensitive solution for the detection of Oncorhynchus keta Genomic DNA Residual (No Internal Control) using advanced real-time PCR technology. With specially designed primers targeting conserved sequences of the Oncorhynchus keta Genomic DNA Residual (No Internal Control) gene, the kit delivers high specificity and real-time fluorescence monitoring for accurate qualitative and quantitative analysis.

Kit Components:
Oncorhynchus keta Genomic DNA Residual (No Internal Control) Amplification Mix: 50 µL × 1 tube
Oncorhynchus keta Genomic DNA Residual (No Internal Control) Reaction Mix: 950 µL × 1 tube
Oncorhynchus keta Genomic DNA Residual (No Internal Control) Positive Control: 50 µL × 1 tube
Oncorhynchus keta Genomic DNA Residual (No Internal Control) Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

No reagent mixing required—just input DNA to begin.
High specificity through design focused on conserved genetic loci.
Excellent signal response with optimized probes and primers.
Built-in positive control ensures dependable results.
Enclosed-tube qPCR minimizes environmental contamination.
Optimized for fast turnaround time.
Not approved for clinical use—research only.

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