Neospora hughesi Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1709-ZJF)

This kit offers a robust and sensitive solution for the detection of Neospora hughesi using advanced real-time PCR technology. With specially designed primers targeting conserved sequences of the Neospora hughesi gene, the kit delivers high specificity and real-time fluorescence monitoring for accurate qualitative and quantitative analysis.

Kit Components:
Neospora hughesi Amplification Mix: 50 µL × 1 tube
Neospora hughesi Reaction Mix: 950 µL × 1 tube
Neospora hughesi Positive Control: 50 µL × 1 tube
Neospora hughesi Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Immediate-use configuration simplifies workflow; DNA is the only input needed.
Sequence specificity ensured by probe design from conserved genomic areas.
Enhanced detection reliability via component refinement.
Includes internal positive standard for reference.
One-tube format reduces handling and contamination risks.
Quick runtime supports efficient processing.
Exclusively for research laboratories.

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