Dusky Spinefoot Iridovirus Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0292-ZJF)

This kit utilizes SYBR Green dye-based real-time fluorescent PCR technology. Specific primers are designed to target highly conserved regions of the Dusky Spinefoot Iridovirus gene. In the presence of the target nucleic acid template, PCR amplification occurs, generating fluorescence signals. The fluorescence intensity is monitored in real time, enabling qualitative and quantitative analysis of Dusky Spinefoot Iridovirus with high sensitivity and specificity.

Kit Components:
Dusky Spinefoot Iridovirus Amplification Mix: 50 µL × 1 tube
Dusky Spinefoot Iridovirus Reaction Mix: 950 µL × 1 tube
Dusky Spinefoot Iridovirus Positive Control: 50 µL × 1 tube
Dusky Spinefoot Iridovirus Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Plug-and-play PCR kit—no extra preparation needed.
High target fidelity thanks to conserved sequence alignment.
Maximum sensitivity with minimized background noise.
Comes with benchmark positive control for result assurance.
One-step fluorescence PCR avoids contamination.
For non-clinical research use only.

STEM-SLSR-0290-ZJF
STEM-SLSR-0291-ZJF
STEM-SLSR-0293-ZJF
STEM-SLSR-0294-ZJF