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Molecular mass analysis of Acetobacter xylinum by Static light scattering (SLS) (CAT#: STEM-MB-0581-WXH)
Introduction
Acetobacter xylinum is a Gram negative soil bacterium that synthesizes and secretes cellulose as part of its metabolism of glucose. Cellulose is the most abundant natural polymer on Earth, and is the major constituent of the cell wall of plants.
Principle
Static light scattering is a technique in physical chemistry that measures the intensity of the scattered light to obtain the average molecular weight Mw of a macromolecule like a polymer or a protein in solution. Measurement of the scattering intensity at many angles allows calculation of the root mean square radius, also called the radius of gyration Rg. By measuring the scattering intensity for many samples of various concentrations, the second virial coefficient, A2, can be calculated.
Applications
The main applications of static light scattering is molecular mass determination of macromolecules, such as proteins and polymers, as it is possible to measure the molecular mass of proteins without any assumption about their shape.
Procedure
1. Sample preparation
2. Measurement by SLS instrument
3. Data analysis
2. Measurement by SLS instrument
3. Data analysis
Materials
• Right-Angle Light Scattering (RALS) Detector
• Low-Angle Light Scattering (LALS) Detector
• Hybrid RALS/LALS Detector
• Multi-Angle Light Scattering (MALS) Detector
• Low-Angle Light Scattering (LALS) Detector
• Hybrid RALS/LALS Detector
• Multi-Angle Light Scattering (MALS) Detector
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