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Isolation of palm tocols by supercritical fluid chromatography (CAT#: STEM-CT-0059-LJX)

Introduction

Crude palm oil contains 600 to 1000 ppm of tocols in the form of tocopherols and tocotrienols. These palm tocols have been isolated and analyzed in the past by various chromatographic techniques such as open column chromatography, high-performance liquid chromatography, as well as thin-layer chromatography. Supercritical fluid chromatography (SFC) has emerged as a more advanced chromatographic technique in recent years. The tocols present in palm oil are successfully isolated using SFC. Identification of these tocols is supported by various spectroscopic techniques such as 1H NMR, 13C NMR, and mass spectrometry.




Principle

Supercritical fluid is a substance that has both gaseous and liquid properties above the critical point. Supercritical fluids have the advantages of high diffusion coefficient, low viscosity, adjustable solubility and high vapor phase density, so they can provide efficient mass spectrometry ionization and separation results.
Supercritical fluid chromatography (SFC) is an efficient separation technique that uses supercritical fluid as a mobile phase. The samples are packed into short tubes or SPE columns, and the samples are compressed and regulated by supercritical fluid to obtain good solubility. The sample components are then separated by column interaction, thus achieving the separation of different compounds.

Applications

For efficient separation of substances
Widely used in biology, chemistry, environmental protection and other fields

Procedure

1. Sample injection
2. The high pressure pump increases the pressure of the sample and mobile phase
3. The sample and mobile phase enter the chromatographic column
4. Flow limiter assists sample separation

Materials

• Sample Type:
Palm tocols

Notes

1. In the process of separation, the control of pressure and temperature of supercritical fluid is very important, which affects the properties and separation efficiency of supercritical fluid.
2. In addition, the selection of the appropriate column, packing and moving equivalent factors will also affect the separation effect.