Iron Skin Dendrobium Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2242-ZJF)

Designed for the detection of Iron Skin Dendrobium, this kit applies probe-based real-time PCR technology. It uses primers targeting conserved gene sequences of Iron Skin Dendrobium, enabling efficient amplification and fluorescent signal generation. Real-time monitoring of fluorescence ensures reliable qualitative and quantitative evaluation of the target nucleic acid.

Kit Components:
Iron Skin Dendrobium Amplification Mix: 50 µL × 1 tube
Iron Skin Dendrobium Reaction Mix: 950 µL × 1 tube
Iron Skin Dendrobium Positive Control: 50 µL × 1 tube
Iron Skin Dendrobium Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Ready-to-use format; users only need to provide sample DNA.
Primers and probes are designed from conserved regions for high specificity.
Optimized oligo sequences deliver excellent sensitivity.
Positive control is included to help identify false-negative results.
Closed-tube, single-step workflow minimizes contamination risk.
Fast detection with minimal processing time.
For research use only.

STEM-SLSR-2240-ZJF
STEM-SLSR-2241-ZJF
STEM-SLSR-2243-ZJF
STEM-SLSR-2244-ZJF